EMBO-PC: Structural Characterization of Macromolecular Complexes
Detailed Programme
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Expression Strategies Chair: Darren Hart Chadwick Amphitheatre
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9:00 - 9.10 |
Welcome |
Carlo Petosa
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9:10 - 9.55 |
Expression strategies using bacterial systems |
Barbara Morris Novagen / EMD Chemicals Madison WI, USA |
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9:55 - 10.40 |
Baculovirus expression strategies for multiprotein complexes |
Imre Berger EMBL Grenoble |
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Coffee |
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11:00 - 11.45 |
Large-scale expression of proteins (and complexes) in mammalian cells |
Radu Aricescu University of Oxford, UK |
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11:45 - 12.30 |
HTP Methods Great and Small high throughput at the brownie scale and beyond |
Christian Kambach Paul Scherrer Institute Villigen, Switzerland |
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Lunch |
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14:00 - 18:00 |
Practicals (Meet in ILL 19/20 hall) |
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18:00 - 19:00 |
Poster clip presentations (EMBL Seminar Room) |
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19:00 - 20:00 |
Dinner (canteen) |
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Isolation of Functional Complexes Chair: Elspeth Gordon Chadwick Amphitheatre
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9:00 - 9:45 |
Strategies for the co-expression of protein complexes in Escherichia coli |
Christophe Romier IGBMC Illkirch, France |
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9:45 - 10:30 |
Strategies for identifying, overproducing and characterizing protein complexes |
Bertrand Séraphin CGM-CNRS Gif-sur-Yvette, France |
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Coffee |
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11:00 - 11:45 |
Production of protein complexes |
Song Tan Penn State University, University Park, PA, USA |
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11:45 - 12:30
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Preparation of recombinant multi-subunit complexes for crystallization |
Chris Oubridge MRC-LMB Cambridge, UK |
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Lunch |
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14:00 - 18:00 |
Practicals |
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18:00 - 19:00 |
Poster Session 1 |
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19:00 - 20:00 |
Dinner (canteen) |
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Characterization of macromolecular complexes Chair: Winfried Weissenhorn Chadwick Amphitheatre
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9:00 - 9:45 |
Structure analysis of biomacromolecular solutions by small-angle X-ray and neutron scattering |
Dmitri Svergun EMBL Hamburg, Germany |
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9:45 - 10:30 |
Analytical
ultracentrifugation and isothermal calorimetry in the study
of protein
complexes |
Vladimir Rybin EMBL Heidelberg, Germany |
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Coffee |
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11:00 - 11:45 |
Tandem Affinity Purification of protein complexes: lessons from a genome-wide screen |
Anne-Claude Gavin EMBL Heidelberg, Germany |
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11:45 - 12:10 |
The
use of size
exclusion chromatography and multi-angle laser light scattering
(MALS) to characterize macromolecular complexes |
Marc Jamin UVHCI Grenoble |
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12:10 - 12:35 |
Analysis
of the conformational dynamics of a protein |
Ricardo Pires EMBL Grenoble |
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Lunch |
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14:00 - 18:00 |
Practicals |
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18:00 - 19:00 |
Guest Lecture (Chadwick Amphitheatre): Protein-protein
interaction modulating ubiquitin and SUMO conjugation |
Titia Sixma NKI Amsterdam, Netherlands |
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19:00 - 20:00 |
Novagen Dinner (ILL Hall 19/20) |
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Electron microscopy & NMR Chair: Guy Schoehn, Chadwick Amphitheatre
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9:00 - 9:45 |
Imaging biological macromolecules
with electron microscopes |
Patrick Schultz IGBMC Illkirch, France |
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9:45 - 10:30 |
Structure determination of dynamic
macromolecular complexes by single particle cryo-EM |
Holger Stark MPI Göttingen, Germany |
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Coffee |
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11:00 - 11:45 |
EM interpretation
with Situs and Sculptor |
Willy Wriggers, D.E. Shaw Research, New York, USA |
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11:45 - 12:30
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Studying
macromolecular complexes by NMR |
Michael Sattler Technische Universität München, Germany |
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Lunch |
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14:00 - 18:00 |
Practicals |
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18:00 - 19:00 |
Poster Session II |
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19:00 - 20:00 |
Dinner (canteen) |
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Strategies in structural and functional analysis Chair: Stephen Cusack, Chadwick Amphitheatre
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9:00 - 9:45 |
Crystallization
strategies
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Song Tan Penn State University, University Park, PA, USA |
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9:45 - 10:30 |
Fluorescence microscopy-based
methods to study macromolecular complexes in live cells |
Jan Ellenberg EMBL Heidelberg |
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Coffee |
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11:00 - 11:45 |
Cryo-EM studies of
co-translational targeting and translocation |
Christiane Schaffitzel EMBL Grenoble |
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11:45 - 12:30
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Structural studies on
yeast transcription system III |
Carlos Fernández-Tornero EMBL Heidelberg |
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Lunch |
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14:00 - 18:00 |
Practicals |
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18:00 - 18:20 |
Feedback session / EMBO Questionnaire |
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approx. 18:30 |
Bus to LeSappey & Banquet |
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More tips & techniques Chair: Carlo Petosa, EMBL Seminar Room
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9:00 - 9:45 |
Synchrotrons,
beamlines, and all that jazz
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Martin Walsh MRC France, BM-14 |
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9:45 - 10:30 |
Mass spectrometry of
noncovalent assemblies |
Frank Sobott University of Oxford, UK |
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10:30 - 11:15 |
Crystallizing
the enhanceosome |
Daniel Panne EMBL Grenoble |
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11:15 - 11:25 |
Final remarks |
Carlo Petosa EMBL Grenoble |