Structural CHARACTERIZATION of MACROMOLECULAR

COMPLEXES, Grenoble, May 15-20, 2006

 

Programme

 

Monday 15 May

 

Expression Strategies

Chadwick Auditorium, ILL   Chair: Darren Hart

 

09:00-9.05

Welcome

Christoph Müller

EMBL Grenoble

09:05-9.50

Practical lessons from a high through-put protein

production pipeline (many clones make light work?)
Download pdf presentation

Nick Berrow

Oxford University

09:50-10.35

Tools for engineering soluble proteins and

finding folded protein domains.

Stephanie Cabantous

Los Alamos National Lab.

10:30-11.00

Coffee

11:00-11.45

Baculovirus expression: history and current practice.
Download pdf presentation

Ian Jones

University of Reading

11:45-12.30

High output mammalian protein production; Large scale transient expression in HEK293 cells.
Download pdf presentation

Wieger Hemrika

University of Utrecht

12:30-13:30

Lunch

 

13:30-14:15

ESRF safety training Groups B and C     -13.30-13.50
                           Groups A, D and E     -13.50-14.15

 

Meeting Point

BM-14

 

14:15-18:00
 
Practicals

 

 

19:00-20:00

Dinner

 

20:00-21:00

Student presentations (EMBL Seminar Room)

followed by refreshments

Bus to Hotel Bastille

at 21.45
 

 

Tuesday 16 May

 

Isolation of Functional Complexes

EMBL Seminar Room   Chair : Carlo Petosa

 

09:00-09:45

Production of protein complexes
Download pdf presentation

Song Tan

Penn State University

09:45-10:30

Preparation of large complexes for crystallisation

Kiyoshi Nagai

MRC-LMB Cambridge

10:30-11:00

Coffee

 

11:00-11:45

Identification, overproduction and characterization of yeast protein complexes using the TAP method
Download pdf presentation

Bertrand Seraphin

CNRS Gif-sur-Yvette

11:45-12:30

 

Crystallography of multicomponent complexes:lessons from RNA polymerase II

 

Michela Bertero

University of Munich

12:45-14:00

Lunch

 

 

14:00-18:00

 

 

Practicals

 

19:00-20:00

Dinner

 

20:00-21:00

Student presentations (EMBL Seminar Room)

followed by refreshments

Bus to Hotel Bastille

At 21.45
 
 
Wednesday 17 May

 

Characterization of Macromolecular Complexes

Chadwick Auditorium, ILL    Chair: Winfried Weissenhorn

 

09:00-09:45

Structure analysis of biomacromolecular

solutions by small-angle X-ray and neutron scattering
Download pdf presentation

Dimitri Svergun

EMBL Hamburg

09:45-10:15

Analytical ultracentrifugation for the study of biological macromolecules
Download pdf presentation

Christine Ebel

IBS, Grenoble

10:15-10:45

Isothermal titration calorimetry in the study of protein complexes
Download pdf presentation

Vladimir Rybin

EMBL Heidelberg

10:45-11:15

Coffee

 

11:15-11:45

"Mass spectrometry for macromolecular complexes"

Min Zhou

Cambridge University

11:45-12:15

Tandem affinity purification of protein

complexes: lessons from a genome-wide screen?
Download pdf presentation

Anne-Claude Gavin

EMBL Heidelberg

12:15-12:45

Structure-based systems biology : modelling protein interactions and complexes
Download pdf presentation

Patrick Aloy

IRB-PCB, Barcelona

12:45-14:00

Lunch

 

 

14:00-18:00
 
Practicals

 

 

18:00-19:00

SAXS demonstration

Dimitri Svergun

 

19.30-20.00

Dinner

Bus to Hotel Bastille

At 20:30
 
 
Thursday 18 May

 

Electron microscopy

Chadwick Auditorium, ILL    Chair:Guy Schoehn

 

09:00-09:45

"Single particle electron microscopy"

Bettina Boettcher

EMBL Heidelberg

09:45-10:30

Structural insights into the splicing machinery
Download pdf presentation

Holger Stark

MPI Göttingen

10:30-11:00

Coffee

 

11:00-11:45

Cellular cryo-electron tomography

Achilles Frangakis

EMBL Heidelberg

11:45-12:30

 

Rigid body and flexible fitting of biophysical data
Download pdf presentation

Willy Wriggers

U. of Texas

12:30-14:00

Lunch

 

 

14:00-18:00
 
Practicals

 

 

19:00-20:00

Dinner

 

20:00-21:00

"Studying macromolecular complexes by NMR"

Michael Sattler

EMBL Heidelberg

21:00

Refreshments

Bus to Hotel Bastille

At 21.45
 
 
Friday 19 May

 

Crystallization/crystallography of macromolecular complexes

EMBL Seminar Room   Chair: Elspeth Gordon

 

09:00-09:30

High-throughput crystallization methods
Download pdf presentation

Jose-Antonio Marquez

EMBL Grenoble

09:30-10:15

Crystallization Strategies
Download pdf presentation

Song Tan

Penn State University

10:15-10:45

Coffee

 

10:45-11:30

"Combining X-ray & EM"

 

Jorge Navaza

IBS Grenoble

11:30-12:15

"Tips and tricks for the structural determination of macromolecular complexes"

Luca Pellegrini

Cambridge University

12:15-12:45

 

Rabies virus NP protein

Winfried Weissenhorn

EMBL Grenoble

12:30-13:30

Lunch

 

 

14:00-18:00

 

Practicals

 

 

18.30

Course Banquet (Le Sappey)

 Bus to Le Sappey at 18.30
 

 

Saturday 20 May

 

X-Ray structure analysis

EMBL Seminar Room   Chair: Christoph Müeller

 

09:00-09:45

"Solving structures by X-ray crystallography"

 

Gordon Leonard

ESRF Grenoble

09:45-10:30

"Structural biology of large complexes"

 

Stephen Cusack

EMBL Grenoble

10:30-11:00

Final remarks

Christoph Müller

EMBL Grenoble

11:00-12:00

Coffee