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Practical
sessions at the
EMBO course on the “Structural Characterisation of Macromolecular
complexes:
Modern Techniques and Strategies, Grenoble, May
3-8, 2004” For every
day except Wednesday 5 different practical sessions have been planned.
From Monday
to Friday the course
participants will be split in 4 groups. On Saturday the
course will be
split in
two groups and we will collect data on two ESRF beam lines following
the
morning lectures. The
following practicals
have been planned: (Click
for detailed
protocols) 1.) T7 transcription 2.)
DNA/protein
complex: Oligopurification/EMSA
of protein 3.) EM negative
staining/particle selection/fitting into EM-densities 4.) Dynamic light
scattering/CD/cross-linking/low-angle scattering 5.)
X-ray data collection at ESRF beamlines a)
ID14-3 b)
BM14 (contact:
Elspeth Gordon, gordon@esrf.fr) The schedule for
the
practicals reads as follows:
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